何化(副教授)

发布者:于道永发布时间:2019-04-24浏览次数:416


何化,博士副教授,硕士研究生导师

教育和工作经历:

201012-至今 中国石油大学化学工程学院副教授

2014/02-2015/02 美国斯坦福大学化学系国家公派访学

2005/09-2008/11 上海交通大学应用化学专业工学博士

1998/09-2002/07湖南师范大学应用化学专业学士

学术兼职:中国化学会会员;ACS Nano, ACS AMI,知名期刊审稿人。

讲授课程和学生指导:本科生课程《生物物理化学》、《生物工程概论》;研究生课程《生物材料》、《生命科学与生物技术进展》;面向留学生的全英文课程《现代仪器分析技术》。指导和协助指导多名博士研究生、国外留学博士生、硕士研究生和本科毕业生,其中,1人获山东省优秀本科毕业论文。


研究方向:

1)   单分子荧光成像、表面增强荧光成像、近红外荧光成像等新成像技术的开发

2)   GPCR等药物靶点分子信号转导机制、活体生物成像诊断和体外肿瘤标志物高灵敏检测

3)   功能荧光纳米探针的合成

获奖情况

2018年青岛市自然科学奖三等奖(排名第5

国家优秀奖学金、香港求是研究生奖学金、“上海交大优秀毕业生”

 

主持科研项目:

1国家自然科学基金面上项目(21874154),《细胞膜上G蛋白偶联受体聚集与分布的荧光闪烁碳点超分辨成像》;

2国家自然科学基金青年项目(20905078),《基于RGD配体设计的CdTe量子点靶向探针的直接合成及其应用》;

3山东省自然基金面上项目(ZR2014BM028),《荧光闪烁量子点用于细胞中趋化因子受体CCR3的三维超分辨定位与分布研究》;

4山东省自然基金青年项目(ZR2009BQ001),《基于RGD肽的量子点靶向探针制备新方法研究及应用》。

5中央高校基本科研业务基金(18CX02126A),《荧光碳纳米材料的制备及其在生物成像中的应用》。

6中央高校基本科研业务基金(12CX04053A),《单个量子点的光学行为、调控及其应用研究》。


英文论著:

(1) Hua He, Xiaojuan Wang, Fang Huang. Analysis and Applications of Single Molecule Fluorescence in Live Cell Membranes, in: ‎Membrane Biophysics: New Insights and Methods, ‎Springer2018, pp 147-173.受邀撰写章节.

以第一作者/通讯作者发表的论文:

Angew. Chem. Int. Ed., Anal. Chem., Nano Lett., Chem. Commun., ACS Appl. Mater. Interfaces 等国际权威SCI刊物发表论文20余篇,总引用达1000次,单篇最高被引用300次。

1.    Hua He, Xinjian Shi, Junying Wang, Xiaojuan Wang, Qian Wang,1 Daoyong Yu, Baosheng Ge, Xiaodong Zhang and Fang Huang. ROS-Induced Aggregation of Nanozyme for Neuron Injury. ACS Appl. Mater. Interfaces 2019, Dec. 16, DOI: 10.1021/acsami.9b17509.

2.    Xiaoyu Mu, Hua He (共同第一作者), Junying Wang, Wei Long, Qifeng Li, Haile Liu, Yalong Gao, Lufei Ouyang, Qinjuan Ren, Si Sun, Jingya Wang, Jiang Yang, Qiang Liu, Yuanming Sun, Changlong Liu, XiaoDong Zhang, Wenping Hu. Carbogenic Nanozyme with Ultrahigh RNS Selectivity for Traumatic Brain Injury. Nano Lett.2019, 19(7), 4527-4534.

3.    Yuan He, Zhenzhen Feng, Xinjian Shi, Shan Li, Yunguo, Liu, Guangming Zeng, and Hua He*(通讯作者), N- and O-doped Carbon Dots for Rapid and High-Throughput Dual Detection of Trace Amounts of Iron in Water and Organic Phases. J. Fluoresc. 2019, 29(1):137-144.

4.    Hua He, Shan Li, Xinjian Shi, Xiaojuan Wang, Xu Liu, Qian Wang, Aijun Guo, Baosheng Ge, Naseer Ullah Khan, and Fang Huang, Quantitative Nanoscopy of Small Blinking Graphene Nanocarriers in Drug Delivery. Bioconjugate Chem. 2018, 29 (11), 3658–3666.

5.    Naseer Ullah Khan, Hua He*(通讯作者), Xiaojuan Wang, Baosheng Ge, Qian Wang, Xu Liu, Jun Lao, Yanan Wang, Jiqiang Li, Zhencai Wang, Shitan Zhou, and Fang Huang. A Two-Color Fluorescence Enhanced Dot-Blot Assay for Revealing Co-operative Expression of Chemokine Receptors in Cells. Chem. Commun. 2018, 54, 778-781.

6.    Naseer Ullah Khan, Zhenzhen Feng, Hua He*(通讯作者), Qian Wang, Xu Liu, Shan Li, Xinjian Shi, Xiaojuan Wang, Baosheng Ge and Fang Huang*, A Facile Plasmonic Silver Needle for Fluorescence-Enhanced Detection of Tumor Markers. Anal. Chim. Acta. 2018, 1040, 120-127.

7.    Lingfang Liu, Xiaoyu Mu, Haixia Liu, Qian Wang, Xueting Bai, Junying Wang , Haile Liu , Fujuan Xu, Yaqi Jing, Haitao Dai, Changlong Liu*, Hua He*(通讯作者), and Xiao-Dong Zhang *, Structure, luminescence, and bioimaging of 2 bimetallic CuAu nanoclusters. Opt. Mater.2018, 86, 291–297.

8.    Hua He, Xu Liu, Shan Li, Xiaojuan Wang, Qian Wang, Jiqiang Li, Junying Wang, Hao Ren, Baosheng Ge, Shengjie Wang, Xiaodong Zhang, Fang Huang, High-Density Super-Resolution Localization Imaging with Blinking Carbon Dots. Anal. Chem.2017, 89 (21), 11831-11838.highlighted in Nat. Meth.

9.    Jun Lao, Hua He (共同第一作者), Xiaojuan Wang, Zhencai Wang, Yanzhuo Song, Bin Yang, Naseer Ullahkhan, Baosheng Ge*, Fang Huang*. Single-Molecule Imaging Demonstrates Ligand Regulation of Oligomeric Status of CXCR4 in Living Cells. J. Phys. Chem. B 2017, 121 (7), 1466-1474.

10.  Hua He, Zhencai Wang, Tiantian Cheng, Xu Liu, Xiaojuan Wang, Junying Wang, Hao Ren, Yawei Sun, Yanzhuo Song, Jiang Yang, Yongqing Xia, Shengjie Wang, Xiaodong Zhang*, and Fang Huang*. Visible and Near-Infrared Dual-Emission Carbogenic Small Molecular Complex with High RNA Selectivity and Renal Clearance for Nucleolus and Tumor Imaging. ACS Appl. Mater. Interfaces 2016, 8 (42), 28529-28537.

11.  Hua He, Xiaojuan Wang, Tiantian Cheng, Yongqing Xia, Jun Lao, Baosheng Ge, Hao Ren, Naseer Ullah Khan, Fang Huang*. An Ultra-High Fluorescence Enhancement and High Throughput Assay for Revealing Expression and Internalization of Chemokine Receptor CXCR4. Chem. Eur. J.2016, 22, 5863-5867.

12.  Hua He*, Xiaojuan Wang, Zhenzhen Feng, Tiantian Cheng, Xing Sun, Yawei Sun, Yongqing Xia, Shengjie Wang, Junying Wang, Xiaodong Zhang*. Rapid microwave-assisted synthesis of ultra-bright fluorescent carbon dots for live cell staining, cell-specific targeting and in vivo imaging. J. Mater. Chem. B2015, 3, 4786-4789.

13.  Hua He, Xing Sun, Xiaojuan Wang, Yawei, Sun, Hai Xu*, Jian R. Lu*. Cyclic Arginyl-Glycyl-Aspartic Acid (RGD) Peptide-Induced Synthesis of Uniform and Stable One-Dimensional CdTe Nanostructures in Aqueous Solution. RSC Adv.2014, 4, 11794-11797.

14.  Hua He*, Xing Sun, Xiaojuan Wang and Hai Xu*, Synthesis of Highly Luminescent and Biocompatible CdTe/CdS/ZnS Quantum Dots Using Microwave Irradiation: a Comparative Study of Different Ligands, Luminescence 2014, 29, 837–845.

15.  Hua He, Min Feng, Jing Hu, Cuixia Chen, Jiqian Wang, Xiaojuan Wang, Hai Xu*, Jian R. Lu*. Designed Short RGD Peptides for One-Pot Aqueous Synthesis of Integrin-binding CdTe and CdZnTe Quantum Dots. ACS Appl. Mater. Interfaces2012, 4, 6362-6370.

16.   Hua He, Jicun Ren*. A Novel Evanescent Wave Scattering Imaging Method for Single Gold Particle Tracking in Solution and on Cell Membrane. Talanta2008, 77(1):166-171.

17.  Hua He, Chao Xie, Jicun Ren*. Non-Bleaching Fluorescence of Gold Nanoparticles and Its Applications in Cancer Cell Imaging. Anal. Chem.2008, 80(15): 5951-5957.JACS等期刊引用221

18.  Hua He, Huifeng Qian, Chaoqing Dong, Kanglin Wang, Jicun Ren*. Single nonblinking CdTe quantum dots synthesized in aqueous thiopropionic acid. Angew. Chem. Int. Ed.2006, 45(45): 7588-7591.Nat. Mater., Phys. Rev. Lett.等顶尖期刊正面引用85

19.  Hua He, Qingji Xie, Shouzhuo Yao*. An electrochemical quartz crystal impedance study on anti-human immunoglobulin G immobilization in the polymer grown during dopamine oxidation at an Au electrode. J. Colloid Interf. Sci.2005, 289, 446-454.

20.  Hua He, Qingji Xie, Youyu Zhang, Shouzhuo Yao*. A simultaneous electrochemical impedance and quartz crystal microbalance study on antihuman immunoglobulin G adsorption and human immunogloulin G reaction. J. Biochem. Bioph. Meth.2005, 62, 191-205.

参与发表的SCI论文:

21.  Haile Liu, Guosong Hong, Zhentao Luo, Junchi Chen, Junlei Chang, Ming Gong, Hua He, Jiang Yang, Xun Yuan, Lulin Li, Xiaoyu Mu, Junying Wang, Wenbo Mi, Jian Luo, Jianping Xie, Xiaodong, Zhang, Atomic-Precision Gold Clusters for NIR-II Imaging, Advanced Materials 2019, 31(46): 1901015.

22.  Wei Long, Junying Wang, Jiang Yang, Hongying Wu, Jingya Wang, Xiaoyu Mu, Hua He, Qiang Liu, Yuanming Sun, Haichao Wang, and Xiaodong Zhang. Naturally-Derived PHA-L Protein Nanoparticle as a Radioprotector Through Activation of Toll-Like Receptor 5. J Biomed. Nanotechnol.2019, 15(1):62-76.

23.  Hao Wang, Xiaoyu Mu, Hua He, Xiao-Dong Zhang,Cancer Radiosensitizers, Trends in Pharmacological Science, 2018, 39, 24-48. (Feature Review).

24.  Xiaojuan Wang, Qi Chen, Zhengyuan Zhang, Hua He, Xiqi Ma, Zhenzhen Liu, Baosheng Ge, Fang Huang, Novel Enteromorpha Prolifera based carbon dots: Probing the radical scavenging of natural phenolic compounds.Colloids Surf., B2018, 174:161-167.

25.  Yanzhuo Song, Baosheng Ge, Jun Lao, Zhencai Wang, Bin Yang, Xiaojuan Wang, Hua He, Jiqiang Li, Fang Huang, Regulation of the Oligomeric Status of CCR3 with Binding Ligands Revealed by Single-Molecule Fluorescence Imaging, Biochemistry2018, 57 (5), 852-860.

26.  Xiaojuan Wang, Yanan Wang, Hua He, Xiqi Ma, Qi Chen, Shuai Zhang, Baosheng Ge, Shengjie Wang, Werner M Nau, Fang Huang. Deep-Red Fluorescent Gold Nanoclusters for Nucleoli Staining: Real-Time Monitoring of the Nucleolar Dynamics in Reverse Transformation of Malignant Cells. ACS Appl. Mater. Interfaces2017, 9 (21), 17799–17806

27.  Xiaojuan Wang, Hua He, Yanan Wang, Junying Wang, Xing Sun, Hai Xu, Werner M Nau*, Xiaodong Zhang*, Fang Huang*. Active TumorTargeting Luminescent Gold Clusters with Efficient Urinary Excretion. Chem. Commun.2016, 52, 9232-9235.

28.  Xiaojuan Wang, Yanan Wang, Hua He, Xin Chen, Xing Sun, Yawei Sun, Guangjun Zhou, Hai Xu, Fang Huang*. Steering graphene quantum dots in living cells: lighting up the nucleolus. J. Mater. Chem. B 2016, 4, 779-784.

29.  Xiaojuan Wang, Xing Sun, Hua He, Hao Yang, Jun Lao, Yanzhuo Song, Yongqing Xia, Hai Xu, Xiaodong Zhang, Fang Huang*. A two-component active targeting theranostic agent based on graphene quantum dots. J. Mater. Chem. B2015, 17, 3583-3590.

30.  Xiaodong Zhang, Jie Chen, Jiang Yang, Jun-Ying Wang, Xiu Shen, ShaSha Song, Hao Wang, Hua He, Xiaojuan Wang, Saijun Fan, Yuanming Sun, Meili Guo. Use of epidermal growth factor receptor antibody–gold cluster conjugates with good renal excretion in targeted cancer radiation treatment. J. Mater. Chem. B2015, 3, 4735-4741.

31.  Xiaojuan Wang, Xing Sun, Jun Lao, Hua He, Tiantian Cheng, Mingqing Wang, Shengjie Wang, Fang Huang*. Multifunctional graphene quantum dots for simultaneous targeted cellular imaging and drug delivery. Colloids Surf., B2014, 122, 638-644.

32.  Yongqing Xia, Xinlong He, Meiwen Cao, Xiaojuan Wang, Yawei Sun, Hua He, Hai Xu*, Jian Ren Lu*. Self-Assembled Two-Dimensional Thermoresponsive Microgel Arrays for Cell Growth/Detachment Control. Biomacromolecules2014, 15, 4021-4031.