何化，博士，副教授
教育和工作经历：
2010年12月-至今 中国石油大学（华东）化学化工学院副教授
2014/02-2015/02 美国斯坦福大学化学系访问学者, 导师：戴宏杰 院士
2008/12-2010/12 中国石油大学（华东）化学化工学院讲师
2005/09-2008/11 上海交通大学应用化学工学博士，导师：任吉存 教授
2002/09-2005/07 湖南师范大学分析化学理学硕士，导师：姚守拙院士
1998/09-2002/07湖南师范大学应用化学学士
学术兼职：Frontiers in Chemistry副主编；山东省物理学会生物物理专委会副秘书长；国家自然科学基金委评议人；中国化学会高级会员；中国光学工程学会会员。
讲授课程和学生指导：本科生课程《仪器分析实验》、《无机及分析实验》、《现代分析技术》、《生物物理化学》、《生物信息学与人工智能》；研究生课程《生物仪器分析技术》、《生物材料》；留学生英文课程《仪器分析技术及应用》。
研究方向：分析化学
1)单分子荧光成像、超分辨荧光成像及人工智能技术的研究及开发
2)重大疾病生物标志物和环境中微塑料等新型污染物的高灵敏检测
获奖情况
2018年青岛市自然科学奖三等奖
国家优秀奖学金、香港求是研究生奖学金、“上海交大优秀毕业生”
科研项目：
1） 国家自然科学基金面上项目（21874154），《细胞膜上G蛋白偶联受体聚集与分布的荧光闪烁碳点超分辨成像》，主持
2） 山东省自然基金面上项目（ZR2023MB010），《基于碳点闪烁的单分子定量超分辨成像新方法及其应用》，主持
3） 山东省自然基金面上项目（ZR2014BM028），《荧光闪烁量子点用于细胞中趋化因子受体CCR3的三维超分辨定位与分布研究》，主持
4） 国家自然科学基金青年项目（20905078），《基于RGD配体设计的CdTe量子点靶向探针的直接合成及其应用》，主持
5） 山东省自然基金青年项目（ZR2009BQ001），《基于RGD肽的量子点靶向探针制备新方法研究及应用》，主持
6） 中央高校基本科研业务基金（18CX02126A），《荧光碳纳米材料的制备及其在生物成像中的应用》，主持
7） 中央高校基本科研业务基金（12CX04053A），《单个量子点的光学行为、调控及其应用研究》，主持
8） 国家重点研发计划（2019YFA0708703），《超浸润智能驱油体系定向输送机制及智能诱导体系研发》，项目骨干
9） 山东省重大研发计划项目（2015GSF118013），《用于重大恶性肿瘤早期预警的等离子体纳米镀膜检测芯片关键技术研究》，第1参加人
10） 海关总署科技项目（2022HK058），《进口原油中非法添加物的识别与安全监管体系研究》，第1参加人
11） 海关总署科技项目（2017IK236），《纳米镀膜等离子体芯片用于石油产品中有毒有害物质的高通量检测研究》，第1参加人
英文论著：
1. Hua He, Qian Wang, Xiaoqiang Wang, Fang Huang. Single molecule fluorescence for studying the membrane protein dynamics and interactions, in: Biomembranomics: Structure, Techniques, and Applications, Jenny Stanford Publishing 2024, Chapter 6, 201-237. 受邀撰写
2. Hua He, Xiaojuan Wang, Fang Huang. Analysis and Applications of Single Molecule Fluorescence in Live Cell Membranes, in: Membrane Biophysics: New Insights and Methods, Springer 2018, Chapter 6, 147-173. 受邀撰写
以第一作者/通讯作者发表的论文：
以第一作者/通讯作者在Science Advances (1篇)、Angewandte Chemie International Edition (1篇)、Nano Letters (4篇)、 Analytical Chemistry (6篇)、ACS Sensors (1篇)、Chemical Communications (3篇)、ACS Applied Materials & Interfaces (3篇)等权威刊物发表论文30余篇, H因子23。
1. Hua He,* Guangyong Qin, Minmin Xue, Zhenzhen Feng, Jian Mao, Wenpeng Tao, Hongqi Chen, Xiaojuan Wang, Daoyong Yu, and Fang Huang*. Mitochondrial Nanoprobe for Precise Cellular and Drug Analysis via Graph Neural Network. Chemical Engineering Journal 2024, 152709.
2. Wenxuan Yang, Shenming Wang, Xiaoyun Wei, Zhenzhen Feng, Guangyong Qin, Jian Mao, Zheng Zhen, Xintong Miao, Yifeng Cheng, Xiaojuan Wang, Fang Huang and Hua He*, Ligand-Regulated Catalytic Activity in Fluorescent Gold Nanoclusters. Inorganic Chemistry Frontiers, 2024, DOI: 10.1039/D4QI01828A
3. Hongqi Chen*, Ruoxin Wang, Juanjuan Cheng, Lun Wang and Hua He*. A Ratiometric Upconversion Nanoprobe Enables Super-Resolution Imaging Sensing of Biothiols in Living Cells. Chemical Communications 2024, 60(33), 4447-4450.
4. Jian Mao, Minmin Xue, Xin Guan, Qian Wang, Zhirui Wang, Guangyong Qin, and Hua He*. Near-Infrared Blinking Carbon Dots Designed for Quantitative Nanoscopy. Nano Letters, 2023, 23, 1, 124-131.
5. Hua He*, Guangyong Qin, Simin Bi, Zhenzhen Feng, Jian Mao, Xin Guan, Minmin Xue, zhirui wang, Xiaojuan Wang, Daoyong Yu, and Fang Huang*. Deep-Learning-Enhanced Diffusion Imaging Assay for Resolving Local-Density Effects on Membrane Receptors. Analytical Chemistry, 2023, 95(6), 3300-3308.
6. Qian Wang, Qian Zhang, Hua He,* Zhenzhen Feng, Jian Mao, Xiang Hu, Xiaoyun Wei, Simin Bi, Guangyong Qin, Xiaojuan Wang, Baosheng Ge, Daoyong Yu, Hao Ren and Fang Huang*. Carbon Dot Blinking Fingerprint Uncovers Native Membrane Receptor Organizations via Deep Learning. Analytical Chemistry 2022, 94(9), 3914-3921.
7. X. Mu#, J. Wang#, Hua He #, Qifeng Li, Bing Yang, Junhui Wang, Haile Liu, Yalong Gao, Lufei Ouyang, Si Sun, Qinjuan Ren, Xinjian Shi, Wenting Hao, Qiaoman Fei, Jiang Yang, Lulin Li, Ryan Vest, Tony Wyss-Coray, Jian Luo, and Xiaodong Zhang*. An oligomeric semiconducting nanozyme with ultrafast electron transfers alleviates acute brain injury. Science Advances 2021, 7(46), eabk1210.
8. Hua He*, Xiaoliang Chen, Zhenzhen Feng, Lihua Liu, Qian Wang, and Simin Bi. Nanoscopic Imaging of Nucleolar Stress Enabled by Protein-Mimicking Carbon Dots. Nano Letters 2021, 21(13), 5689–5696.
9. Naseer Ullah Khan, Muhammad Zahir, Xukun Liu, Jing Lin, Qihong Zheng, Huajie Zhang, Samiullah Malik, Hua He *, and Liming Shen*. Ultra-sensitive detection of exosome using biofunctionalized gold nanorods on silver-island film. Nano Letters 2021, 21(13), 5532–5539.
10. Qian wang, Hua He *, Qian Zhang, Zhenzhen Feng, Jiqiang Li, Xiaoliang Chen, Lihua Liu, Xiaojuan Wang, Baosheng Ge, Daoyong Yu, Hao Ren and Fang Huang*. Deep-Learning-Assisted Single-Molecule Tracking on a Live Cell Membrane. Analytical Chemistry 2021, 93(25), 8810–8816
11. Hua He, Lihua Liu, Xiaoliang Chen, Qian wang, Xiaojuan Wang, Werner M. Nau*, and Fang Huang*. Carbon Dot Blinking Enables Accurate Molecular Counting at Nanoscale Resolution. Analytical Chemistry 2021, 93(8), 3968–3975.
12. Qian wang, Zhenzhen Feng, Hua He*, Jiqiang Li, Xiaoliang Chen, Lihua Liu, Xiaojuan Wang, Baosheng Ge, Daoyong Yu, Hao Ren and Fang Huang*. Nonblinking Carbon Dots for Imaging and Tracking Receptors on Live Cell membrane. Chemical Communications 2021, 57(45), 5554-5557.
13. Hua He*, Di Liu, Zhenzhen Feng, Aijun Guo, Lihua Liu, and Xiaoliang Chen. Antifade Carbon Dots on Plasmonic Substrate for Enhanced Protein Detection in Immunotherapy. ACS Sensors 2020, 5(12), 4027–4034.
14. Hua He, Xinjian Shi, Junying Wang, Xiaojuan Wang, Qian Wang, Daoyong Yu, Baosheng Ge, Xiaodong Zhang* and Fang Huang*. Reactive Oxygen Species-Induced Aggregation of Nanozymes for Neuron Injury. ACS Applied Materials & Interfaces 2020, 12(1), 209-216.
15. Lufei Ouyang, Xiaoyu Mu, Junying Wang, Qifeng Li, Yalong Gao, Haile Liu, Si Sun, Qinjuan Ren, Ruijuan Yan, Jingya Wang, Qiang Liu, Yuanming Sun, Changlong Liu, Hua He*, Wei Long and Xiaodong Zhang*. Carbon dot targeting to nitrogen signaling molecules for inhibiting neuronal death. Journal of Materials Chemistry B 2020, 8(11), 2321-2330.
16. Xiaoyu Mu#, Hua He#, Junying Wang#, Wei Long, Qifeng Li, Haile Liu, Yalong Gao, Lufei Ouyang, Qinjuan Ren, Si Sun, Jingya Wang, Jiang Yang, Qiang Liu, Yuanming Sun, Changlong Liu, Xiaodong Zhang*, Wenping Hu. Carbogenic Nanozyme with Ultrahigh RNS Selectivity for Traumatic Brain Injury. Nano Letters 2019, 19(7), 4527-4534.
17. Yuan He, Zhenzhen Feng, Xinjian Shi, Shan Li, Yunguo, Liu*, Guangming Zeng, and Hua He* , N- and O-doped Carbon Dots for Rapid and High-Throughput Dual Detection of Trace Amounts of Iron in Water and Organic Phases. Journal of Fluorescence 2019, 29(1):137-144.
18. Naseer Ullah Khan, Hua He*, Xiaojuan Wang, Baosheng Ge, Qian Wang, Xu Liu, Jun Lao, Yanan Wang, Jiqiang Li, Zhencai Wang, Shitan Zhou, and Fang Huang*. A Two-Color Fluorescence Enhanced Dot-Blot Assay for Revealing Co-operative Expression of Chemokine Receptors in Cells. Chemical Communications 2018, 54, 778-781.
19. Hua He, Shan Li, Xinjian Shi, Xiaojuan Wang, Xu Liu, Qian Wang, Aijun Guo, Baosheng Ge, Naseer Ullah Khan, and Fang Huang, Quantitative Nanoscopy of Small Blinking Graphene Nanocarriers in Drug Delivery. Bioconjugate Chemistry 2018, 29 (11), 3658–3666.
20. Naseer Ullah Khan, Zhenzhen Feng, Hua He*, Qian Wang, Xu Liu, Shan Li, Xinjian Shi, Xiaojuan Wang, Baosheng Ge and Fang Huang*, A Facile Plasmonic Silver Needle for Fluorescence-Enhanced Detection of Tumor Markers. Analytica Chimica Acta 2018, 1040, 120-127.
21. Lingfang Liu, Xiaoyu Mu, Haixia Liu, Qian Wang, Xueting Bai, Junying Wang, Haile Liu, Fujuan Xu, Yaqi Jing, Haitao Dai, Changlong Liu*, Hua He*, and Xiaodong Zhang *, Structure, luminescence, and bioimaging of bimetallic CuAu nanoclusters. Optical Materials 2018, 86, 291–297.
22. Hua He, Xu Liu, Shan Li, Xiaojuan Wang, Qian Wang, Jiqiang Li, Junying Wang, Hao Ren, Baosheng Ge, Shengjie Wang, Xiaodong Zhang, Fang Huang, High-Density Super-Resolution Localization Imaging with Blinking Carbon Dots. Analytical Chemistry 2017, 89 (21), 11831-11838.
23. Jun Lao#, Hua He#, Xiaojuan Wang, Zhencai Wang, Yanzhuo Song, Bin Yang, Naseer Ullahkhan, Baosheng Ge*, Fang Huang*. Single-Molecule Imaging Demonstrates Ligand Regulation of Oligomeric Status of CXCR4 in Living Cells. Journal of Physical Chemistry B 2017, 121 (7), 1466-1474.
24. Hua He, Zhencai Wang, Tiantian Cheng, Xu Liu, Xiaojuan Wang, Junying Wang, Hao Ren, Yawei Sun, Yanzhuo Song, Jiang Yang, Yongqing Xia, Shengjie Wang, Xiaodong Zhang*, and Fang Huang*. Visible and Near-Infrared Dual-Emission Carbogenic Small Molecular Complex with High RNA Selectivity and Renal Clearance for Nucleolus and Tumor Imaging. ACS Applied Materials & Interfaces 2016, 8 (42), 28529-28537.
25. Hua He, Xiaojuan Wang, Tiantian Cheng, Yongqing Xia, Jun Lao, Baosheng Ge, Hao Ren, Naseer Ullah Khan, Fang Huang*. An Ultra-High Fluorescence Enhancement and High Throughput Assay for Revealing Expression and Internalization of Chemokine Receptor CXCR4. Chemistry-A European Journal 2016, 22, 5863-5867.
26. Hua He *, Xiaojuan Wang, Zhenzhen Feng, Tiantian Cheng, Xing Sun, Yawei Sun, Yongqing Xia, Shengjie Wang, Junying Wang, Xiaodong Zhang*. Rapid microwave-assisted synthesis of ultra-bright fluorescent carbon dots for live cell staining, cell-specific targeting and in vivo imaging. Journal of Materials Chemistry B 2015, 3, 4786-4789.
27. Hua He, Xing Sun, Xiaojuan Wang, Yawei, Sun, Hai Xu*, Jian R. Lu*. Cyclic Arginyl-Glycyl-Aspartic Acid (RGD) Peptide-Induced Synthesis of Uniform and Stable One-Dimensional CdTe Nanostructures in Aqueous Solution. RSC Advances 2014, 4, 11794-11797.
28. Hua He*, Xing Sun, Xiaojuan Wang and Hai Xu*, Synthesis of Highly Luminescent and Biocompatible CdTe/CdS/ZnS Quantum Dots Using Microwave Irradiation: a Comparative Study of Different Ligands, Luminescence 2014, 29, 837–845.
29. Hua He, Min Feng, Jing Hu, Cuixia Chen, Jiqian Wang, Xiaojuan Wang, Hai Xu*, Jian R. Lu*. Designed Short RGD Peptides for One-Pot Aqueous Synthesis of Integrin-binding CdTe and CdZnTe Quantum Dots. ACS Applied Materials & Interfaces 2012, 4, 6362-6370.
30. Hua He, Chao Xie, Jicun Ren*. Non-Bleaching Fluorescence of Gold Nanoparticles and Its Applications in Cancer Cell Imaging. Analytical Chemistry 2008, 80(15): 5951-5957.
31. Hua He, Jicun Ren*. A Novel Evanescent Wave Scattering Imaging Method for Single Gold Particle Tracking in Solution and on Cell Membrane. Talanta 2008, 77(1):166-171.
32. Hua He, Huifeng Qian, Chaoqing Dong, Kanglin Wang, Jicun Ren*. Single nonblinking CdTe quantum dots synthesized in aqueous thiopropionic acid. Angewandte Chemie International Edition 2006, 45(45): 7588-7591.
33. Hua He, Qingji Xie, Shouzhuo Yao*. An electrochemical quartz crystal impedance study on anti-human immunoglobulin G immobilization in the polymer grown during dopamine oxidation at an Au electrode. Journal of Colloid and Interface Science 2005, 289, 446-454.
34. Hua He, Qingji Xie, Youyu Zhang, Shouzhuo Yao*. A simultaneous electrochemical impedance and quartz crystal microbalance study on antihuman immunoglobulin G adsorption and human immunogloulin G reaction. J. Biochem. Bioph. Meth. 2005, 62, 191-205.
国家发明专利：
1) 一种双效荧光增强的免疫分析方法, 专利号：ZL 202010520800.1
2) 一种受自由基诱导聚集的治疗性纳米酶及其制备方法，专利号：ZL201910660001.1
3) 一种基于免疫荧光增强的外泌体定量检测方法, 专利号：ZL202110648316.1